Solubilization of NAD(P)-independent sorbitol dehydrogenase ofGluconobacter oxydanssubspeciessuboxydansusing octyl-glucoside
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منابع مشابه
Nucleotide Sequence of a cDNA Encoding NADP-Sorbitol-6-Phosphate Dehydrogenase from Apple.
Sorbitol plays a key role in the translocation of photosynthate in the Rosaceae family, including apple (Malus domestica), pear, and stone fruits (4, 7). NADP-S6PDH2 plays the most important role in biosynthesis of sorbitol in apple because its activity increases before accumulation of sorbitol in seedlings (8), and high activity is maintained in leaves (10). This enzyme reduces G6P to S6P usin...
متن کاملSolubilization studies and properties of particulate NAD-independent malate dehydrogenase of Acinetobacter calcoaceticus (N.C.I.B. 8250).
Erythrocytes from the same mouse strains gave similar results; here the cell number was increased to 109/ml to obtain a significant difference between curves for highand low-responder mice. It is possible that these results are affected by contamination with mouse ferritin from the spleen or plasma despite washing of cells. To minimize possible contamination by mouse ferritin, lymph-node cells,...
متن کاملSynthesis of Octyl-β-Glucoside Catalyzed by Almond β-Glucosidase in Unconventional Reaction Media.
The reaction system for octyl-β-glucoside synthesis catalysed by the almond-β-glucosidase has been characterised. The monophasic octanol saturated with different amounts of water served as a reaction medium. Both the glucose and the activated substrate p-nitrophenyl-β-glucoside were examined as glycon donors. The reverse hydrolysis and the transglycosylation were both used as reaction models fo...
متن کاملStructural phase transitions involved in the interaction of phospholipid bilayers with octyl glucoside.
The transitional stages induced by the interaction of the nonionic surfactant octyl glucoside (OcOse) on phosphatidylcholine liposomes were studied by means of transmission electron microscopy (TEM), light scattering and permeability changes. A linear correlation was observed between the effective surfactant/lipid molar ratio (Re; three-stage model proposed for liposome solubilization) and the ...
متن کاملSorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana.
Sorbitol is converted to fructose in Rosaceae species by SORBITOL DEHYDROGENASE (SDH, EC 1.1.1.14), especially in sink organs. SDH has also been found in non-Rosaceae species and here we show that the protein encoded by At5g51970 in Arabidopsis thaliana (L.) Heynh. possesses the molecular characteristics of an SDH. Using a green fluorescent protein-tagged version and anti-SDH antisera, we deter...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1983
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1983.tb00462.x